ICSI Treatment In India
ICSI (Intra Cytoplasmic Sperm Injection) – An Injection Of Hope
ICSI – Is It For Us?
Before making this crucial choice, the two of you should consider the following questions:
If so, ICSI may be the answer you’ve been seeking for.
How Does the ICSI Procedure Work ?
The only difference between ICSI and IVF is the method of fertilisation. In IVF, the fertilisation process takes place in a disc, but in ICSI, each sperm is individually injected into each egg using a device known as a micromanipulator.
What Are The Steps Leading Up To ICSI?
What Are The ICSI Success Rates?
The success rates of more than 800 of these ICSI Procedures performed at Dr. Nandita Palshetkar‘s IVF facilities around India were estimated to be 40%, which is an injection of much-needed hope.
Males with extremely low counts (less than 5 million per ml) or poor quality sperm had little chance of having children until the 1990s. The new ICSI discovery, which occurred in 1992 in Brussels, Belgium, solved this issue.
Since then, numerous patients of this type have fathered children. Since 1995–1996 when we began our own ICSI programme, we have completed more than 800 cycles with a success rate of 30–40%, which is equivalent to the finest units worldwide.
All of the steps in ICSI are comparable to those in IVF, with the exception of the fertilisation phase. One egg is often combined with 100,000 sperms during IVF, and one sperm fertilises the egg on its own. In ICSI, however, a single live sperm is kept and injected into each egg. The Micromanipulator, a device, is used to perform this micro-fertilization.
The Infertility India clinic offers thorough, reasonably priced ICSI therapy and services. ICSI Center in India, our facility provides ICSI treatment for infertility.
Males with sperm anti-bodies
Men who have ejaculated dysfunction from a spinal cord disorder or injury, such as paraplegics or quadriplegics.
Patients who attempt IUI but are unsuccessful due to retrograde ejaculation (ejection of sperm into the bladder).
Patients in whom in vitro fertilisation has not resulted in pregnancy.
In our clinic, we also think that using ICSI on patients with a history of endometriosis or TB is a good idea because we think it results in higher fertilisation rates than traditional IVF (this is a personal experience not supported by international literature).
For all patients, including those with normal sperm counts, several units now advocate routine ICSI. As we feel that pregnancy should be achieved with the least amount of handling of the gametes outside the body, we do not support this procedure. We won’t perform ICSI if the sperm count is sufficient for IVF fertilisation. However, if a patient’s sperm count is in the grey area, we can choose to use IVF for half the eggs and ICSI for the other half.
Our success rates, which are similar to the best in the world for both azoospermia and non-azoospermia patients, are in the range of 30 to 40%.
ICSI uses a different method of fertilisation than IVF. ICSI is a process where each egg is held and injected with a single live sperm, in contrast to IVF when one egg is combined with one lakh sperms, with fertilisation taking place on its own. The Micromanipulator, a device, is used to perform this micro-fertilization. There are 11 steps in the technique (anchor).
Controlled Ovarian stimulation with drugs (GnRH Analogues and Gonadotrophins) to produce many eggs.
Monitoring of follicles and egg development with the aid of vaginal sonography and serial estradiol hormone estimation. Administration of hCG injection, (Human Chorionic Gonadotrophins) when the two leading follicles are 18mm. in diameter. Oocyte or egg retrieval under short general anaesthesia, 35 to 37 hours after HCG injection.
Laboratory isolation and identification of eggs
sperm collecting and laboratory processing. When there are no sperm in the semen (azoospermia), the sperm are taken directly from the testis using the PESA/MESA/FTNB/TESE or TESA procedures.
the laboratory dissection of the eggs using the enzyme hyloronetis putting eggs into tiny culture media droplets submerged in oil.
Sperms are placed in tiny PVP droplets that are submerged in oil. Using a micro-injection needle with a 7 micron diameter to immobilise the sperm and aspirate it into the needle (tail first).
Using a holding pipette to hold the egg while injecting the immobilised sperm into the held egg these eggs are put into the incubator for two to five days. 2 to 5 days after fertilisation, embryos begin to develop.
after two days (four cell embryo), three days (six to eight cell embryo), or five days (blastocyst stage), good quality embryos are transferred back into the womb.